在妊娠过程中，胚胎是“种子”，子宫内膜是“土壤”。

　　种子再好，若土壤“贫瘠”，也难以孕育生命。

　　那么该如何精准评估子宫内膜状态?

　　深圳中山妇产医院全国首家生殖免疫实验室，用数字化技术揭开子宫内膜免疫的奥秘，为反复种植失败、复发性流产患者带来新希望!

　　为什么子宫内膜免疫检测如此重要?

　　子宫内膜中的免疫细胞(如NK细胞、巨噬细胞、T细胞等)就像“土壤的守护者”，通过调控免疫平衡，促进血管形成和滋养层侵袭等，直接影响胚胎着床和发育。

　　然而这些免疫细胞在子宫内膜中过多或过少，均可影响局部的免疫微环境平衡，导致“土壤”过于肥沃或者贫瘠，从而诱发移植失败或反复流产。但传统评估方法(如超声、病理染色及血液检测)主观性强、准确性低，难以精准反应子宫局部的免疫状态。

　　然而即使检测子宫局部免疫细胞，当前临床检测也存在两大痛点：

　　1️⃣ 缺乏定量指标，医生“凭经验”判断各类细胞含量;

　　2️⃣ 各机构检测标准不统一，结果难以参考。

　　那么这时候又该怎么办呢?

　　全国首创!数字化免疫分析平台“破局”

　　深圳中山妇产医院历时十余年，建立国内首个子宫内膜免疫细胞数字化分析平台，数字化检测让子宫内膜免疫的问题一目了然，为治疗提供精准方向。

　　从检测到精准实现三大突破：

　　1.测得准

　　(1) 筛选4大类免疫细胞、7项关键指标(如CD56+NK细胞、CD68+巨噬细胞、CD8+T细胞，Foxp3+调节性T细胞等)，首次绘制子宫内膜免疫细胞全景图谱。

　　(2) 引进AI全景定量系统，从人工计数升级为全切片数字化分析，人员分析准确性及一致率可达90%以上，报告周期从3周缩短至1周。

　　2.有标准

　　(1) 根据国际临床实验室标准，建立中国可孕女性子宫内膜免疫参考区间，填补领域空白。(2) 研究成果被国内外指南引用，获发明专利5项、科技进步奖6项。

　　3.强有效

　　(1) 临床验证显示：通过免疫调控，反复种植失败患者妊娠率提升至56%，复发性流产保胎率达92%。

　　(2) 已服务超10000家庭，覆盖全国18个省市，44%样本来自外院委托!

　　未来展望: 让更多家庭“怀得上、保得住”

　　实验室持续升级技术，发起多中心临床研究共同探索子宫内膜免疫细胞检测的临床意义，推动行业标准建立。未来将通过大数据模型进一步优化诊疗路径，让精准医疗惠及更多患者。

　　从“经验医学”到“数字医学”，深圳中山妇产医院用创新技术破解生殖难题。如果您正面临反复移植失败或流产困扰，子宫内膜免疫评估或许是解开谜题的关键!

       下附 已发表SCI论文(近3年代表作)：

　　1. Yahyaei A, Madani T, Vesali S, Mashayekhi M. Intrauterine infusion of autologous platelet rich plasma can be an efficient treatment for patients with unexplained recurrent implantation failure. Sci Rep. 2024 Oct 29;14(1):26009.

　　2. Zhang H, Diao L. Editorial: Recurrent pregnancy loss and endocrine dysfunction. Front Endocrinol (Lausanne). 2024 Jul 9;15:1453336.

　　3. Zhang Y, Yang L, Yang D, Cai S, Wang Y, Wang L, Li Y, Li L, Yin T, Diao L. Understanding the heterogeneity of natural killer cells at the maternal-fetal interface: implications for pregnancy health and disease. Mol Hum Reprod. 2024 Nov 14;30(11):gaae040.

　　4. Hong L, Xiao S, Diao L, Lian R, Chen C, Zeng Y, Liu S. Decreased AMPK/SIRT1/PDK4 induced by androgen excess inhibits human endometrial stromal cell decidualization in PCOS. Cell Mol Life Sci. 2024 Jul 30;81(1):324.

　　5. Cao Y, Yang D, Cai S, Yang L, Yu S, Geng Q, Mo M, Li W, Wei Y, Li Y, Yin T, Diao L. Adenomyosis-associated infertility: an update of the immunological perspective. Reprod Biomed Online. 2025 May;50(5):104703.

　　6. Yang L, Su Y, Cai S, Ma H, Yang J, Xu M, Li Y, Huang C, Zeng Y, Li Q, Feng M, Li H, Diao L. Regional Analysis of the Immune Microenvironment in Human Endometrium. Am J Reprod Immunol. 2024 Sep;92(3):e13921.

　　7. Wang L, Yin Z, Shen Y, Feng G, Dai F, Yang D, Deng Z, Yang J, Chen R, Yang L, Chen X, Sun Q, Huang C, Cheng Y, Deng H, Diao L, Li L, Yin T. Targeting Decidual CD16+ Immune Cells with Exosome-Based Glucocorticoid Nanoparticles for Miscarriage. Adv Sci (Weinh). 2024 Aug 13:e2406370.

　　8. Chen C, Huang C, Wu Y, Li Z, Yu S, Chen X, Lian R, Lin R, Diao L, Zeng Y, Li Y. Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis. J Vis Exp. 2023 Oct 13;(200).

　　9. Zhang H, Qiu H, Liu Z, Yu S, Chen C, Zeng Y, Li Y. Endometrial preparation protocols did not impact pregnancy outcomes of patients with cured chronic endometritis. Reprod Biomed Online. 2024 Jun;48(6):103753.

　　10. Zhang F, Wang Z, Lian R, Diao L, Li Y, Wu Y, Yin T, Huang C. Intrauterine perfusion of dexamethasone improves pregnancy outcomes in recurrent reproductive failure patients with elevated uterine natural killer cells. A retrospective cohort study. Am J Reprod Immunol. 2023 Dec;90(6):e13796.

　　11. Jin F, Liu W, Cheng G, Cai S, Yin T, Diao L. The function of decidua natural killer cells in physiology and pathology of pregnancy. Am J Reprod Immunol. 2023 Sep;90(3):e13755.

　　12. Yang J, Wang L, Ma J, Diao L, Chen J, Cheng Y, Yang J, Li L. Endometrial proteomic profile of patients with repeated implantation failure. Front Endocrinol (Lausanne). 2023 Jul 31;14:1144393.

　　13. Yu S, Diao L, Lian R, Chen C, Huang C, Li X, Li Y, Zeng Y. Comparing the peri-implantation endometrial T-bet/GATA3 ratio between control fertile women and patients with recurrent miscarriage: establishment and application of a reference range. Hum Reprod. 2023 Sep 5;38(9):1680-1689.

　　14. Cai S, Dai S, Lin R, Huang C, Zeng Y, Diao L, Lian R, Tu W. The effectiveness and safety of intrauterine infusion of autologous regulatory T cells (Tregs) in patients with recurrent pregnancy loss and low levels of endometrial FoxP3+ cells: A retrospective cohort study. Am J Reprod Immunol. 2023 Aug;90(2):e13735.

　　15. Yu S, Lian R, Chen C, Chen X, Xu J, Zeng Y, Li Y. Impact of body mass index on peripheral and uterine immune status in the window of implantation in patients with recurrent reproductive failure. Hum Fertil (Camb). 2023 Dec;26(5):1322-1333.

　　16. Yu S, Huang C, Lian R, Diao L, Zhang X, Cai S, Wei H, Chen C, Li Y, Zeng Y. Establishment of reference intervals of endometrial immune cells during the mid-luteal phase. J Reprod Immunol. 2023 Mar;156:103822.

　　17. Li Y, Xu Y, Yu S, Lin S, Chen W, Lian R, Diao L, Sun H, Ding L, Zeng Y. Chronic endometritis impairs embryo implantation in patients with repeated implantation failure: A retrospective study. Taiwan J Obstet Gynecol. 2022 Nov;61(6):984-988.

　　18. Ma H, Cai S, Yang L, Wang L, Ding J, Li L, Li H, Huang C, Diao L. How do pre-pregnancy endometrial macrophages contribute to pregnancy? J Reprod Immunol. 2022 Dec;154:103736.

　　19. Wang L, Deng Z, Yang J, Zhao Y, Zhou L, Diao L, Li L, Cheng Y. Epigenetic and transcriptomic characterization of maternal-fetal interface in patients with recurrent miscarriage via an integrated multi-omics approach. J Reprod Immunol. 2022 Dec;154:103754.

　　20. Cai S, Lin R, Liu S, Wang X, Wei H, Huang C, Li Y, Chen X, Li L, Zeng Y, Lian R, Diao L. Intrauterine infusion of human chorionic gonadotropin improves the endometrial FoxP3+ Tregs level and pregnancy outcomes in patients with lower endometrial FoxP3+ Tregs. J Reprod Immunol. 2022 Sep;153:103678.

　　21. Chen X, Qi L, Zhao C, Xue J, Chen M, Diao L, He W, Lv B, Zeng Y, Xue Z. Decreased expression of SEMA4D in recurrent implantation failure induces reduction of trophoblast invasion and migration via the Met/PI3K/Akt pathway. J Reprod Immunol. 2022 Sep;153:103657.

　　22. Li Y, Yu S, Huang C, Diao L, Chen C, Liu W, Lian R, Mo M, Du C, Liu F, Zeng Y. Predictive role of endometrial T-bet/GATA3 ratio during mid-luteal phase for live birth in patients undergoing in vitro fertilization: A retrospective observational study. J Reprod Immunol. 2022 Feb;149:103465.

　　23. Liu S, Hong L, Lian R, Xiao S, Li Y, Diao L, Zeng Y. Transcriptomic Analysis Reveals Endometrial Dynamics in Normoweight and Overweight/Obese Polycystic Ovary Syndrome Women. Front Genet. 2022 May 13;13:874487.

　　24. Jia Y, Dong YJ, Sha YL, Cai SC, Diao LH, Qiu Z, Guo YH, Huang Y, Ye HX, Liu S. Effectiveness comparison between endometrial receptivity array, immune profiling and the combination in treating patients with multiple implantation failure. Am J Reprod Immunol. 2022 Mar;87(3):e13513.